Composite

Part:BBa_K5048011:Design

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-13)


T7-lac operator-lptE(full length)-FLAG-dt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We design this part in order to reproduce the result of literature, except for the double terminators. we discussed with CDG China and they recommended this part because it had high efficiency.



Source

The LptE comes from the genomic sequence of E.coli K-12 strain. T7 promoter and Lac operator comes from the backbone. double terminators comes from iGEM parts collection

References